Journal: Cell reports

Article Title: Molecular cascade reveals sequential milestones underlying hippocampal neural stem cell development into an adult state

doi: 10.1016/j.celrep.2024.114339

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-Nfix , Thermo Fisher Scientific , Cat# PA5–30897; RRID: AB_2548371.

Techniques: Recombinant, Electron Microscopy, Fluorescence, Reverse Transcription, Plasmid Preparation, Flow Cytometry, Software

Journal: bioRxiv

Article Title: Discovering cancer stem-like cells using Spatial transcriptomic analysis: Nuclear factor I X as a novel therapeutic target for gastric cancer

doi: 10.1101/2024.03.31.587468

Figure Lengend Snippet: Identification of NFIX as a key gene associated with cancer stemness in gastric cancer. (A) Gene Ontology enrichment analysis of the top 50 genes of cancer stem cell-like cells. (B) The overlap of the Venn diagram showed that there are 16 candidate targeted genes predicted by Cluster 0, Monocle3, and CytoTRACE. (C) Feature plots of each candidate gene. (D) Spatial plot of NFIX.

Article Snippet: The sections were incubated with rabbit polyclonal anti- NFIX antibody (NBP2-15039, 1:200; Novus Biologicals, LLC, USA) for 1 h at room temperature, followed by incubation with EnVision+ peroxidase-conjugated anti-rabbit secondary antibody for 1 h. For the color reaction, the sections were incubated with the Dako Liquid DAB+ Substrate Chromogen System (#K3468, Santa Clara, CA, USA) for 3 min.

Techniques:

Journal: bioRxiv

Article Title: Discovering cancer stem-like cells using Spatial transcriptomic analysis: Nuclear factor I X as a novel therapeutic target for gastric cancer

doi: 10.1101/2024.03.31.587468

Figure Lengend Snippet: Correlation of NFIX expression with poor prognosis in gastric cancer (GC). (A-D) Representative immunohistochemical images of NFIX. (A and B) Immunohistochemical staining of NFIX in non-neoplastic gastric mucosa. Original magnification: (A) 100×; scale bars, 200 µm and (B) 400×; scale bars, 50 µm. (C and D) Immunohistochemical staining of NFIX in GC. Original magnification: (C) 100×; scale bars, 200 µm and (D) 400×; scale bars, 50 µm. (E) Overall survival probability in the 127 GC cases. (F) Overall survival probability in the public RNA-seq dataset.

Article Snippet: The sections were incubated with rabbit polyclonal anti- NFIX antibody (NBP2-15039, 1:200; Novus Biologicals, LLC, USA) for 1 h at room temperature, followed by incubation with EnVision+ peroxidase-conjugated anti-rabbit secondary antibody for 1 h. For the color reaction, the sections were incubated with the Dako Liquid DAB+ Substrate Chromogen System (#K3468, Santa Clara, CA, USA) for 3 min.

Techniques: Expressing, Immunohistochemical staining, Staining, RNA Sequencing

Journal: bioRxiv

Article Title: Discovering cancer stem-like cells using Spatial transcriptomic analysis: Nuclear factor I X as a novel therapeutic target for gastric cancer

doi: 10.1101/2024.03.31.587468

Figure Lengend Snippet:

Article Snippet: The sections were incubated with rabbit polyclonal anti- NFIX antibody (NBP2-15039, 1:200; Novus Biologicals, LLC, USA) for 1 h at room temperature, followed by incubation with EnVision+ peroxidase-conjugated anti-rabbit secondary antibody for 1 h. For the color reaction, the sections were incubated with the Dako Liquid DAB+ Substrate Chromogen System (#K3468, Santa Clara, CA, USA) for 3 min.

Techniques: Expressing

Journal: bioRxiv

Article Title: Discovering cancer stem-like cells using Spatial transcriptomic analysis: Nuclear factor I X as a novel therapeutic target for gastric cancer

doi: 10.1101/2024.03.31.587468

Figure Lengend Snippet: Role of NFIX in gastric cancer (GC) cells: proliferation, stemness, and kinase activity regulation. (A) Western blot analysis of NFIX in five non-neoplastic or GC cell lines. (B) Western blot analysis of NFIX in MKN-45 cells transfected with the negative control or NFIX siRNA. (C) Effect of NFIX knockdown on cell growth in MKN-45 cells transfected with the negative control or NFIX siRNA. (D and E) Wound-healing assay in MKN-45 cells transfected with the negative control or NFIX siRNA. (D) The mean percentage of wound closure. (E) Representative image. (F) Number and size of spheroids formed by MKN-45 cell lines transfected with negative control or NFIX siRNA. (G) The heatmap shows the gene expression profile of 3 independent sets of MKN-45 cells transfected with the negative control or NFIX siRNA by RNA sequencing analysis. Data are color-coded to reflect the gene expression level. (H) Volcano plot comparing MKN-45 cells transfected with the negative control and that with NFIX siRNA. (I) Gene Ontology enrichment analysis of the top 50 genes.

Article Snippet: The sections were incubated with rabbit polyclonal anti- NFIX antibody (NBP2-15039, 1:200; Novus Biologicals, LLC, USA) for 1 h at room temperature, followed by incubation with EnVision+ peroxidase-conjugated anti-rabbit secondary antibody for 1 h. For the color reaction, the sections were incubated with the Dako Liquid DAB+ Substrate Chromogen System (#K3468, Santa Clara, CA, USA) for 3 min.

Techniques: Activity Assay, Western Blot, Transfection, Negative Control, Knockdown, Wound Healing Assay, Gene Expression, RNA Sequencing

Journal: bioRxiv

Article Title: Discovering cancer stem-like cells using Spatial transcriptomic analysis: Nuclear factor I X as a novel therapeutic target for gastric cancer

doi: 10.1101/2024.03.31.587468

Figure Lengend Snippet: Proliferative capacity and stemness in non-gastric cancer cell lines. (A) Western blot analysis of NFIX in HFE-145 cells transfected with the negative control or NFIX siRNA. (C) Effect of NFIX knockdown on cell growth in HFE-145 cells transfected with the negative control or NFIX siRNA. (C and D) Wound-healing assay in HFE-145 cells transfected with the negative control or NFIX siRNA. (C) The mean percentage of wound closure. (D) Representative image. (E) Number and size of spheroids formed by MKN-45 cell lines transfected with negative control or NFIX siRNA.

Article Snippet: The sections were incubated with rabbit polyclonal anti- NFIX antibody (NBP2-15039, 1:200; Novus Biologicals, LLC, USA) for 1 h at room temperature, followed by incubation with EnVision+ peroxidase-conjugated anti-rabbit secondary antibody for 1 h. For the color reaction, the sections were incubated with the Dako Liquid DAB+ Substrate Chromogen System (#K3468, Santa Clara, CA, USA) for 3 min.

Techniques: Western Blot, Transfection, Negative Control, Knockdown, Wound Healing Assay

Journal: bioRxiv

Article Title: Discovering cancer stem-like cells using Spatial transcriptomic analysis: Nuclear factor I X as a novel therapeutic target for gastric cancer

doi: 10.1101/2024.03.31.587468

Figure Lengend Snippet: Confirmation of genetic variation using gastric cancer cell lines. (A) Heatmap of genes found to be related in RNA sequence. Quantitative reverse transcription-polymerase chain reaction analysis of NFIX, RASSF2, MIR21, STRADB and ADARA2A genes in MKN-45 cells transfected with the negative control or NFIX siRNA.

Article Snippet: The sections were incubated with rabbit polyclonal anti- NFIX antibody (NBP2-15039, 1:200; Novus Biologicals, LLC, USA) for 1 h at room temperature, followed by incubation with EnVision+ peroxidase-conjugated anti-rabbit secondary antibody for 1 h. For the color reaction, the sections were incubated with the Dako Liquid DAB+ Substrate Chromogen System (#K3468, Santa Clara, CA, USA) for 3 min.

Techniques: Sequencing, Reverse Transcription, Polymerase Chain Reaction, Transfection, Negative Control

Journal: iScience

Article Title: MEK-inhibitors decrease Nfix in muscular dystrophy but induce unexpected calcifications, partially rescued with Cyanidin diet

doi: 10.1016/j.isci.2023.108696

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Nfix , Geneka , Cat# 16021118.

Techniques: Muscles, Recombinant, DC Protein Assay, SYBR Green Assay